The hepatitis E virus (HEV) is a small non-enveloped RNA virus that belongs to the genus Hepevirus in the family Heperviridae. HEV has known to be 4 genotypes but only one serotype. Genotype 1 and 2 infect only humans and Genotype 3 and 4 infect humans, pigs and other animal species. In Korea, several human HEV infections due to the consumption of infected wild animals have been reported. Pig and wild boars (Susscrofa) are known as reservoirs of HEV infection. The aim of this study was to investigate the nationwide seroprevalenceagainstHEV infection and the genetic characterization of HEV among Korean wild boars (Susscrofa) from 2011 to 2016.Of the 1,399 wild boars tested, serum samples from 505 (36.1%; 95% CI: 33.6-38.6) were seropositive for HEV, which were detected using ELISA. The highest rate was 42.7% (148/346) in 2016. Twenty four HEV strains were identified in a total 1,860 wild boar bloods collected from 2015 to 2016, which indicated a 1.3% rate of HEV viremia prevalence.The phylogenetic tree constructed based on the common 412-nt sequence within ORF2 revealed that among the 24 Korean wild boar HEV strains obtained in the present study, the 23 Korean wild boar HEV strains belonged to Genotype 4 showing the nucleotide identities 83.4-100%. The 19 strains of them were further segregated into subgenotype 4a (88.3-100%) and other 4 strains were segregated into subgenotype 4d (95.8-100%). The one Korean wild boar HEV strain belonged to Genotype 3, segregated into subgenotype 3a. These results suggest that HEV genotype 3a, genotype 4a and 4d are circulating in wild boars in Korea and have potential risk factor for HEV transmission to human.